<p>HEK293T (A-C), U2OS (D-F) and PC-12 (G-I) cells expressing wild type (WT) or mutant VCP/p97 (P137L) were co-transfected with LAMP1-RFP and colocalization was analyzed by confocal microscopy under basal/non-starved (NON-STV) (A, D, G) or starved (STV) (B, E, H) conditions. Overexpressed MYC-VCP was indirectly immunostained using anti-MYC antibodies. Arrowheads indicate colocalized dots in (A,B),(D,E) and (G,H). Quantification of co-localized dots in HEK293T (C), U2OS (F) and PC-12 (I) cells. Minimum 40 cells were analyzed under each condition, and quantification was expressed as a percentage of colocalized positive cells within the total transfected cell population. Data were shown as mean ± SD of independent experiments (n = 3). * p<0.05...
(A) The autophagosome marker GFP:Atg8a was expressed in control, w- or vap1 fat bodies using a cg-Ga...
<p>(A) HeLa cells were infected with Sch-MeV (MOI 1). At the indicated time points post infection, c...
<p>iSLK-BAC16 and iSLK-BAC-K297R cells were induced with Dox (3 μg/ml) for 3 days for viral lytic re...
<p>(A-C) WT or mutant VCP/p97 constructs were co-transfected to HEK293T cells with GFP-tagged LC3 an...
<p>(A) Primary structure of VCP/p97 protein and IBMPFD-related mutations. Major protein domains were...
<p>EGFP ULK2 WT or its mutants (P242A, P794A) were transfected in HEK293 cells as described in the M...
<p>A, Surface expression of mSlo1 and TRPV1. HEK293 cells were cotransfected with mSlo1-TagGFP (gree...
<p><b>(A-C),</b> Confocal micrographs of U2OS cells transfected with mRFP-EEA1 (A), GFP-Rab7 (B), or...
Note that exogenous mRFP-Vps34 was comparable to endogenous Vps34 and stable during amino acid starv...
<p>(A) Confocal microscopy analysis of control and VMP1-depleted HeLa cells expressing the omegasome...
<p>Live-cell confocal microscopic images of AdGFP-LC3 (green) and lysosomes (red) stained by LysoTra...
<p>To visualize co-localization of EGFP-ULK2 WT, S1027A, and S1027D (green) with endogenous human At...
<p><b>(A)</b> The nuclear extracts of HEK-293T cells transiently expressing the indicated FOXC1 muta...
<p>MDCK cells were transfected with cDNA encoding GFP-VAMP7 (A) or with control or VAMP7 siRNA (B) a...
<p>(A) Cells were infected with WT and M1 viruses for indicated time points and cell lysates were an...
(A) The autophagosome marker GFP:Atg8a was expressed in control, w- or vap1 fat bodies using a cg-Ga...
<p>(A) HeLa cells were infected with Sch-MeV (MOI 1). At the indicated time points post infection, c...
<p>iSLK-BAC16 and iSLK-BAC-K297R cells were induced with Dox (3 μg/ml) for 3 days for viral lytic re...
<p>(A-C) WT or mutant VCP/p97 constructs were co-transfected to HEK293T cells with GFP-tagged LC3 an...
<p>(A) Primary structure of VCP/p97 protein and IBMPFD-related mutations. Major protein domains were...
<p>EGFP ULK2 WT or its mutants (P242A, P794A) were transfected in HEK293 cells as described in the M...
<p>A, Surface expression of mSlo1 and TRPV1. HEK293 cells were cotransfected with mSlo1-TagGFP (gree...
<p><b>(A-C),</b> Confocal micrographs of U2OS cells transfected with mRFP-EEA1 (A), GFP-Rab7 (B), or...
Note that exogenous mRFP-Vps34 was comparable to endogenous Vps34 and stable during amino acid starv...
<p>(A) Confocal microscopy analysis of control and VMP1-depleted HeLa cells expressing the omegasome...
<p>Live-cell confocal microscopic images of AdGFP-LC3 (green) and lysosomes (red) stained by LysoTra...
<p>To visualize co-localization of EGFP-ULK2 WT, S1027A, and S1027D (green) with endogenous human At...
<p><b>(A)</b> The nuclear extracts of HEK-293T cells transiently expressing the indicated FOXC1 muta...
<p>MDCK cells were transfected with cDNA encoding GFP-VAMP7 (A) or with control or VAMP7 siRNA (B) a...
<p>(A) Cells were infected with WT and M1 viruses for indicated time points and cell lysates were an...
(A) The autophagosome marker GFP:Atg8a was expressed in control, w- or vap1 fat bodies using a cg-Ga...
<p>(A) HeLa cells were infected with Sch-MeV (MOI 1). At the indicated time points post infection, c...
<p>iSLK-BAC16 and iSLK-BAC-K297R cells were induced with Dox (3 μg/ml) for 3 days for viral lytic re...