Successful elimination of non-neural cells and unachievable elimination of glial cells by means of commonly used cell culture manipulations during differentiation of GFAP and SOX2 positive neural progenitors (NHA) to neuronal cells-1

Tiation. (a) Results of Real time PCR for relative expression presented as mean of relative expression and respective standard error. (b) Products of Real time PCR for cDNA visualized on agarose gel electrophoresis. (c) Detection of levels of MAP2 protein expression by Western blot analysis. RNA and protein was isolated from particular cell populations (1, non-neural cells; 2, undifferentiated cells; 3, population consists of neural and non-neural cells; 4, neural cells). MW, molecular weight marker.<p><b>Copyright information:</b></p><p>Taken from "Successful elimination of non-neural cells and unachievable elimination of glial cells by means of commonly used cell culture manipulations during differentiation of GFAP and SOX2 positive neural progenitors (NHA) to neuronal cells"</p><p>http://www.biomedcentral.com/1472-6750/8/56</p><p>BMC Biotechnology 2008;8():56-56.</p><p>Published online 19 Jul 2008</p><p>PMCID:PMC2488339.</p><p>