mRNA expression levels for classical neural genes such as GFAP, NESTIN and NEUROFILAMENTS, investigated with RT-PCR on BM-MSCs, CB-MSCs, he-MSCs and AF-MSCs, after 2, 6 and 10 D.I.V. after neural trans-differentiation treatment.

<p>Panel 1. Light Microscopy images at 40X magnification of BM-MSC (1a-c), CB-MSC (3a-c), hE-MSC (5a-c) AF-MSC (7a-c) at 2 D.I.V a), 6 D.I.V. b), 10 D.I.V, c), in presence of neural differentiation factors as reported in the section: ‘Materials and Methods’. Panel 2. Expression of neural-like cells specific markers in differentiated BM-MSC (2a-c), CB-MSC (4a-c), hE-MSC (6a-c) AF-MSC (8a-c) at 10 D.I.V. assessed by immunostaining for a) GFAP, b) Nestin, c) Neurofilaments, respectively.