Phagocytosis of haemozoin (malarial pigment) enhances metalloproteinase-9 activity in human adherent monocytes: Role of IL-1beta and 15-HETE-3

Er 3 h phagocytosis and a further incubation during 48 h (HZ-fed monocytes) or 48 h after addition of 15-HETE, IL-1beta levels were measured by ELISA in cell supernatants. Data are given as ng IL-1beta/ml supernatant (mean values ± SD of four independent experiments). . After 3 h phagocytosis and a further incubation during 48 h (HZ-fed monocytes) or 48 h after addition of 15-HETE, cell supernatants were separated by PAGE and MMP-9 enzyme activity measured by gelatin zymography and densitometric quantification (see legend to Figure 2 for details). The 83-kDa negative bands in the gel correspond to MMP-9 enzyme activity. Data are given as arbitrary densitometric units (mean values ± SD of four independent experiments). Data were analysed for significance by Student's t-test. Significance of differences (column/lane numbers). Unfed(1) vs HZ-fed(5) monocytes, p < 0.05; unfed(1) vs 15-HETE-treated (3,4,5) monocytes, p < 0.05 (Panel A) and p < 0.01 (Panel B).<p><b>Copyright information:</b></p><p>Taken from "Phagocytosis of haemozoin (malarial pigment) enhances metalloproteinase-9 activity in human adherent monocytes: Role of IL-1beta and 15-HETE"</p><p>http://www.malariajournal.com/content/7/1/157</p><p>Malaria Journal 2008;7():157-157.</p><p>Published online 18 Aug 2008</p><p>PMCID:PMC2529304.</p><p>