<p>(<b>A</b>) C57MG cells were pre-treated with either luciferase or pooled Ctnnb1 siRNA reagent for 24 h prior to additional 24 h incubation with BSA or Rspo2 (10 ng/ml) plus Wnt3a (100 ng/ml). Total and soluble β-catenin and Hsp70 were detected as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029455#pone-0029455-g004" target="_blank">Fig. 4</a> legend. (<b>B–D</b>) Quantitative PCR analysis of <i>Axin2</i>, <i>Ahr</i> and <i>CXCR6</i> expression by C57MG cells after 24 h incubation with luciferase or pooled Ctnnb1 siRNA reagent followed by 24 h treatment with BSA or Rspo2 plus Wnt3a [experiment performed in parallel with (A), using the same reagent concentrations]. Results are means +/− S.D. of tripli...
<p><i>A</i>, Expression of Wnt/-catenin target genes (eGFP reporter and <i>Axin2</i>) and the AR tar...
<p>Data for Figure 2b. Measured β-catenin dependent firefly: constitutive renilla ratios for HT1080,...
<p>T-47D cells were treated for 24 hrs with siRNA against β-catenin (<b>A</b>) or FZD7 (<b>B</b>). E...
<p>(<b>A</b>) Immunoblot analysis of total β-catenin (cell lysate, 30 µg protein/lane) and soluble β...
<p>(<b>A</b>) Quantitative RT-PCR analysis of <i>Angpt1</i> and <i>CCL7</i> expression following 24 ...
<p>(A) Luciferase assay showing transcriptional activity of a β-catenin dependent TCF/LEF4 reporter ...
<p>CSCs were transfected with a siRNA against the β-catenin transcript. The siRNA-mediated transfect...
<p>(<b>A</b>) Efficient knockdown of endogenous β-catenin in HEK-293 and SW480 cells. Total RNA was ...
<p>(A) Inhibition of the β-catenin/Tcf/Lef1-responsive luciferase reporter gene by PI compounds. The...
<p>Quantitative RT-PCR analyses demonstrate that in (A) primary mouse embryonic fibroblasts and (B) ...
<p>C57MG cells were treated with BSA, Rspo2 (100 ng/ml), Wnt3a (100 ng/ml) alone or in combination f...
<p>(<b>A</b>) Quantitative RT-PCR analysis of specific gene expression in response to Rspo2 (100 ng/...
<p>(A) One representative western blot used to quantify p38-siRNA interference efficiency and β-cate...
<p>A) Representative results of immunofluorescence of β-catenin localization in SW480 cells using β-...
<p>(A) Immunocytochemical staining for β-catenin protein on cultured ARβ1 cells reveals loss of β-ca...
<p><i>A</i>, Expression of Wnt/-catenin target genes (eGFP reporter and <i>Axin2</i>) and the AR tar...
<p>Data for Figure 2b. Measured β-catenin dependent firefly: constitutive renilla ratios for HT1080,...
<p>T-47D cells were treated for 24 hrs with siRNA against β-catenin (<b>A</b>) or FZD7 (<b>B</b>). E...
<p>(<b>A</b>) Immunoblot analysis of total β-catenin (cell lysate, 30 µg protein/lane) and soluble β...
<p>(<b>A</b>) Quantitative RT-PCR analysis of <i>Angpt1</i> and <i>CCL7</i> expression following 24 ...
<p>(A) Luciferase assay showing transcriptional activity of a β-catenin dependent TCF/LEF4 reporter ...
<p>CSCs were transfected with a siRNA against the β-catenin transcript. The siRNA-mediated transfect...
<p>(<b>A</b>) Efficient knockdown of endogenous β-catenin in HEK-293 and SW480 cells. Total RNA was ...
<p>(A) Inhibition of the β-catenin/Tcf/Lef1-responsive luciferase reporter gene by PI compounds. The...
<p>Quantitative RT-PCR analyses demonstrate that in (A) primary mouse embryonic fibroblasts and (B) ...
<p>C57MG cells were treated with BSA, Rspo2 (100 ng/ml), Wnt3a (100 ng/ml) alone or in combination f...
<p>(<b>A</b>) Quantitative RT-PCR analysis of specific gene expression in response to Rspo2 (100 ng/...
<p>(A) One representative western blot used to quantify p38-siRNA interference efficiency and β-cate...
<p>A) Representative results of immunofluorescence of β-catenin localization in SW480 cells using β-...
<p>(A) Immunocytochemical staining for β-catenin protein on cultured ARβ1 cells reveals loss of β-ca...
<p><i>A</i>, Expression of Wnt/-catenin target genes (eGFP reporter and <i>Axin2</i>) and the AR tar...
<p>Data for Figure 2b. Measured β-catenin dependent firefly: constitutive renilla ratios for HT1080,...
<p>T-47D cells were treated for 24 hrs with siRNA against β-catenin (<b>A</b>) or FZD7 (<b>B</b>). E...