β-catenin knockdown recapitulates the affect of SIRT1/2 inhibition on FZD7.

<p>T-47D cells were treated for 24 hrs with siRNA against β-catenin (<b>A</b>) or FZD7 (<b>B</b>). Expression of related genes (β-catenin, FZD7, and GAPDH) were measured by real-time PCR. Gene expression was normalized to GAPDH. <b>B</b>. T-47D cells were transfected with siRNA against either β-catenin or FZD7 for 48-hours using oligofectamine, cells were processed for Western blot analysis probing for β-catenin and FZD7. An * indicates a p-value <0.05 versus control. Samples were normalized to non-immune IgG, (n = 3).