Comparison of gene expression profiles of CD4+ T cells transduced with WT Foxp3, K17R, K18R or EV.

<p>(<b>A</b>) Venn diagrams summarizing overlapping upregulated (left) or downregulated (right) gene expression profiles of CD4+ T cells transduced with WT Foxp3 (blue), K17R (purple) or K18R (yellow) compared with CD4+ T cells transduced with EV (cutoff was set as Log2 fold >1). (<b>B–E</b>) Heat maps showing gene expression profiles of CD4+ T cells transduced with EV, WT Foxp3, K17R or K18R. (<b>B</b>) Heat maps showing distinct Treg ‘signature’ gene expression profiles. (<b>C</b>) Heat maps indicating the change in gene expression profiles of Treg cell identified and putative suppressive genes. (<b>D</b>) Heat maps indicating the distinct gene expression profiles of Foxp3 directly bound genes. (<b>E</b>) Heat maps indicating distinct gene expression profiles of Treg-related cytokine genes. (<b>F</b>) qPCR assays of Treg-specific genes selected from microarray analyses; results are representative of 3 independent experiments, and *p<0.05, **p<0.01 compared to WT Foxp3.