Semi-quantitative dot-blot analysis of 103Q-GFP and 25Q-GFP.

<p>Transformants of 74-D694 [<i>psi</i>⁻] [<i>PIN</i>⁺] or [<i>psi</i>⁻] [<i>pin</i>⁻] with the multicopy p103Q-GFP or p25Q-GFP plasmids were grown as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029832#s4" target="_blank">Materials and Methods</a> with incubation in SC-Ura Gal medium for indicated time. Equal amounts of total protein (confirmed by staining the same membranes by Ponceau S, a non-specific protein stain) from each lysate were serially diluted in four-fold increments and applied to a nitrocellulose membrane. Blots were probed with the monoclonal anti-GFP antibody.