P42 reduces aggregate formation of polyQ-hHtt in HeLa cells.

<p>HeLa cells were transfected with GFP-138Q-hHtt^171aa (detected in red), in the presence of: A- empty vector; B- Cherry-P4^166aa; or C- Cherry-P42^23aa expressing vectors detected by the Cherry tag (in green). DAPI staining is shown in grey. D- Quantification of aggregation by dot-blot filtration retardation assay on cells transfected with GFP-138Q-hHtt^171aa, in the presence of empty vector (-), 0Q-hHtt^548aa, P4^166aa or P42^23aa as indicated. Aggregates were detected with an anti-GFP antibody. Quantification was performed on 3 independent experiments and is reported on a graph as percentages with respect to the control (-) set up at 100%. Data were analyzed by using the <i>Student’s t-test</i>: <i>***p<0.001</i>. The presence of hHtt covering either 548 aa, 166 aa or 23 aa leads to equivalent rescues that are not significantly different (NS).