The effect of mutation in <i>CaGRF10</i> and <i>CaPHO4 on</i> the morphology and lethal effect of prototypic strains BWP17 and SN152.

<p>(A,B) Abundant filamentation is produced by <i>pho4</i>Δ on low phosphate solid medium (A) but not on high phosphate (B). (C) The percentage of filamented colonies in prototypic strains and its mutants <i>grf10</i>Δ and <i>pho4</i>Δ. n = 10 microscopy fields with 20-100 colonies/field, The number of filamented colonies on low Pi medium was significantly higher in SC5314, BWP17, and <i>pho4</i>Δ, *p<0.01, and in BWP17 low Pi as compared to <i>grf10</i>Δ low Pi, **p<0.01. On high phosphate medium, the number of filaments was significantly higher in <i>pho4</i>Δ compared to SN152, ***p<0.01. (D) Kaplan-Meyer survival curves in <i>C. elegans</i>. n = 70/variant, p<0.01 in between <i>pho4</i>Δ and SN152 induced mortality in low Pi medium. (E) Kaplan-Meyer survival curves in mice subjected hepatectomy and cecal injection of BWP17 or its derivative <i>grf10</i>Δ. n = 10/variant, *p<0.01.