Effect of temperature and genotoxic agents on PS1 hairpin mutants.

<p>(A) Cultures of yeast strains BY4741 (wild-type), MDY225 (<i>mcm2_K633A</i>), MDY220 (<i>mcm4_K658A</i>), MDY222 (<i>mcm5_K506A</i>), MDY256 (<i>mcm6_K665A</i>), and MDY254 (<i>mcm7_K550A</i>) were grown overnight in YPD at 30°C, serially diluted 10-fold, spotted onto YPD plates and incubated at either 16°C, 30°C or 37°C. (B) Cultures of yeast strains BY4741 (wild-type), MDY225 (<i>mcm2_K633A</i>), MDY220 (<i>mcm4_K658A</i>), MDY222 (<i>mcm5_K506A</i>), MDY256 (<i>mcm6_K665A</i>), and MDY254 (<i>mcm7_K550A</i>) were grown in YPD, and 10-fold serial dilutions spotted onto YPD and YPD containing either 0.03% methyl methanesulfonate (MMS) or 200 mM hydroxyurea (HU). Plates were incubated at 30°C. A <i>mec2-1 </i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0082177#pone.0082177-Weinert1" target="_blank">[51]</a> strain, known to be sensitive to genotoxic stress, was also spotted on the plates.