Scheme of the dsRBDs of <i>Mj</i>-TRBP and the SDS-PAGE analysis of recombinant dsRBDs of <i>M</i>j-TRBP and <i>Fc</i>-eIF6 expressed in <i>E. coli</i>.

<p>The N-terminal (first), middle (second), and C-terminal (third) dsRBDs were denoted as dsRBD A, dsRBD B, and dsRBD C, respectively. The recombinant protein TRBP-DA, TRBP-DB, TRBP-DC, TRBP-DAB, and TRBP-DBC contain dsRBD A, dsRBD B, dsRBD C, dsRBD A and dsRBD B, and dsRBD B and dsRBD C,respectively. Lane M, molecular mass marker; lane 1, total protein obtained from <i>E. coli</i>; lane 2, total protein obtained from expressing strains induced by IPTG; lane 3, recombinant proteins purified by His-Bind resin chromatography.