Evaluation of discrimination ability at different temperatures.

<p>(A) Fluorescence emission spectra of P_HIV (50 nM) at different conditions: (a) P_HIV-RCPN complex; (b) P_HIV-RCPN complex + 300 nM T₁; (c) P_HIV-RCPN complex + 300 nM T₂; (d) P_HIV-RCPN complex + 300 nM T₃; (e) P_HIV-RCPN complex + 300 nM T₄. Inset: fluorescence intensity histograms with error bar. (B) Fluorescence signal enhancement of P_HIV-RCPN complex upon incubation with T₁ and T₂ at 25 and 50°C, respectively. Excitation was at 480 nm, and the emission was monitored at 518 nm. All measurements were done in Tris-HCl buffer in the presence of 15 mM Mg²⁺ (pH: 7.4). The error bar represents the standard deviation of three measurements.