BCCIP knock-down increased apoptosis in the mitotic proliferating region of <i>BCCIP-CKD</i> neocortices.

<p>(A) βIII-tubulin staining identifies the differentiating neural cell populations. Analysis of apoptosis was done by cleaved-caspase 3 (c-C3) staining in E14.5 using <i>BCCIP-CON</i> and <i>BCCIP-CKD</i> embryos. The merged composites are overlay of βIII-tubulin and DPAI staining. (<b>B</b>) Ki67 staining identifies the proliferating mitotic region. Analysis of apoptosis was done by cleaved-caspase 3 (c-C3) staining in E14.5 using <i>BCCIP-CON</i> and <i>BCCIP-CKD</i> embryos. (<b>C</b>) Apoptosis was analyzed by TUNEL assay in E14.5 using <i>BCCIP-CON</i> and <i>BCCIP-CKD</i> embryos. (<b>D</b>) Quantification of TUNEL staining. The amount of apoptosis was quantified in the VZ/SVZ of E14.5 embryos. (<b>E</b>) Quantification of cleaved-caspase 3 staining. The amount of apoptosis was quantified in the VZ/SVZ of E14.5 embryos. CP: cortical plate. VZ/SVZ: ventricular zone/subventricular zone. White bars: <i>BCCIP-CON</i>; Gray bars: <i>BCCIP-CKD</i>. The merged composites are overlay of Ki67 and DAPI staining. CP: cortical plate. VZ/SVZ: ventricular zone/subventricular zone. *: P<0.05; **: P<0.01; ***: P<0.001.