Defining ambiguity.

<p>Molt4 cells were stained for phospho-S10-histone H3 (PHH3-A488), cyclin A2 (Cyclin A2-A647), and DNA content. A: typical view for counting mitotic cells (green dots). This view provides ambiguous data vis-à-vis profile extraction since the cells between the G2 cluster and the bulk of mitotic events at the highest PHH3 levels represent cells that were in the processes of net gain and net loss of PHH3 (A, dual arrows). The ambiguity can be resolved by plotting PHH3 versus cyclin A2 (B). Arrows show the direction of movement through the data space of cell traversing the cell cycle.