Segmentation of multiparametric data.

<p>Samples from the same Molt4 population as shown in previous figures were stained for cyclins A2, B1, PHH3, and DNA content. Segmentation is more complex then single cyclin stained samples, requiring four bivariate views. The first segment (labeled “1”) begins in A (cyclin A2 vs. PHH3) then jumps to B, which is a plot of cyclin A2 vs. cyclin B1 for interphase cells (NOT gated for mitotic cells identified by a region (not shown) set as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030870#pone.0030870.s001" target="_blank">Figure S1</a>), and begins with “2” and ends with “21”. The next segment is back in A, labeled “22” and ends at “34”. The next segment, “35” is in C, which is a plot of cyclin A2 vs. cyclin B1 for mitotic cells (AND gated for mitotic cells as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030870#pone.0030870.s001" target="_blank">Figure S1</a>). The final cluster is segmented in a bivariate of PHH3 vs. cyclin B1 (not shown). D shows resolution of the last mitotic cluster by color (cyan, magenta), labeled “39” and “40” in a 3D plot of PHH3, cyclin A2, and cyclin B1 to show the orthogonal nature of the segment continuum for mitotic cells.