Effects of HFru and HFat feeding on glucose tolerance and hepatic insulin signal transduction.

<p>The experiments were performed after one week of high fructose (HFru, ▪), high fat (HFat, □) or chow (CH, •) feeding. (<b>A</b>) Glucose tolerance test (GTT) was performed with an injection of glucose (3 g/kg, <i>ip</i>) after 5–7 hours of fasting. Data are mean ± SE, 6–10 mice per group. iAUC, incremental area under the curve for blood glucose level. Insulin signal transduction was assessed by immunoblotting of phosphorylated-Akt (Ser473) (<b>B</b>) and -GSK3β (Ser219) (<b>C</b>) in the liver in response to a bolus of insulin stimulation (2 U/kg, <i>ip</i>). Each lane represents a single mouse. Densitometry data are mean ± SE of 6 mice per group. ** <i>p</i><0.01.