Cells coming from a <i>cdc14-1</i> release frequently form Rad52 repair factories, which accumulate when rDNA missegregation had previously occurred.

<p>(A) Strains FM531 (<i>cdc15-2 RAD52-YFP</i>) and FM515 (<i>cdc14-1 RAD52-YFP</i>) were treated as in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002509#pgen-1002509-g002" target="_blank">Figure 2</a>. Cells from samples taken every 30′ were scored (>200 cells each) for number of Rad52-YFP foci (mean ± SEM, n = 3). (B) Time-lapse fluorescence microscopy (every 15–30′ for 6 h) of a FM515 (<i>cdc14-1 RAD52-YFP</i>) cell starting at the time of the telophase release. (C) Strain FM551 (<i>cdc14-1 RAD52-YFP tetO:rDNA tetR-mRFP</i>) was first arrested in the <i>cdc14-1</i> block and then released into a new cell cycle. After 2 hours, Rad52 foci were scored for those foursomes that have either segregated or missegregated the rDNA (mean ± SEM, n = 3). (D) A representative micrograph of two foursomes, one showing segregated <i>tetOs</i> (white triangles) and the other one with unresolved <i>tetOs</i> (the black triangle). Note the Rad52 focus near the unresolved <i>tetOs</i>. In the merged micrograph, mRFP is pseudocoloured in blue and DAPI in red.