Immunofluorescence analysis of Rad51 foci in Pol β proficient and deficient cells after MMS treatment

<p><b>Copyright information:</b></p><p>Taken from "The accumulation of MMS-induced single strand breaks in G phase is recombinogenic in DNA polymerase β defective mammalian cells"</p><p>Nucleic Acids Research 2005;33(1):280-288.</p><p>Published online 12 Jan 2005</p><p>PMCID:PMC546155.</p><p>© 2005, the authors © </p> Wild-type and Pol β cells were treated with 0.5 mM MMS for 15 min and then allowed to repair for the indicated periods of time. The spatial distribution of Rad51 foci is shown in representative nuclei of wild-type () and Pol β () cells under high magnification. The histogram () reports the percentage of Rad51 foci. Cells with at least 10 foci were counted as Rad51 positive cells