Adipocyte-specific deletion of PTP1B has no effect on body mass, adiposity or food intake.

<p><i>A</i>: Deletion efficiency of PTP1B in fl/fl controls (FL) and adip-crePTP1B^−/− mice (KO), as detected by immunoblotting. Tissues shown, (left to right) are brown adipose tissue (BAT), white adipose tissue (WAT), isolated adipocytes (Adips), muscle, liver, brain, bone marrow derived macrophages (BMDM) and intraperitoneal macrophages (Perit Mφ). <i>B</i>: Weight curves for fl/fl (<i>n</i> = 14), adip-cre (<i>n</i> = 6), adip-crePTP1B^+/− (<i>n</i> = 5) and adip-crePTP1B^−/− mice (<i>n</i> = 4) on chow diet for 21 weeks from weaning. <i>C</i>: Weight curves for fl/fl (<i>n</i> = 13), adip-cre (<i>n</i> = 6), adip-crePTP1B^+/− (<i>n</i> = 5) and adip-crePTP1B^−/− mice (<i>n</i> = 4) on HFD diet for 21 weeks. <i>D</i>: Fat pad weight of mice on HFD for 21 weeks. Tissues shown, (left to right) are epididymal (EPI), peri-renal (RENAL) and brown adipose tissue (BAT). Fl/fl (<i>n</i> = 13); adip-cre (<i>n</i> = 6); adip-crePTP1B^+/− (<i>n</i> = 5); adip-crePTP1B^−/− (<i>n</i> = 4). <i>E</i>: Daily food intake of mice on chow or HFD. fl/fl (FL) (chow <i>n</i> = 5, HFD <i>n</i> = 7); adip-crePTP1B^−/− (KO) (chow <i>n</i> = 4, HFD <i>n</i> = 5). <i>F</i>: Increased epididymal adipocyte cell size in chow-fed adip-crePTP1B^−/− mice (KO) compared with adip-crePTP1B^+/− mice (HET), fl/fl (FL) and cre-alone controls (CRE). <i>G</i>: Increased epididymal adipocyte cell size in HFD-fed adip-crePTP1B^−/− mice (KO) compared with adip-crePTP1B^+/− mice (HET), fl/fl and cre-alone controls (FL/CRE). <i>H</i>: Hematoxylin and eosin stained epididymal white adipose tissue of mice on chow or HFD diet for 21 weeks. <i>n</i> = 4 mice/group. White circles = fl/fl; white squares = adip-cre; black circles = adip-crePTP1B^+/−; black squares = adip-crePTP1B^−/−. White bars = fl/fl; diagonally striped bars = adip-cre; horizontally striped bars = adip-crePTP1B^+/−; black bars = adip-crePTP1B^−/−. Data are represented as mean ± SEM. Data were analyzed using two-tailed Student's t test (**<i>P</i><0.01).