Claudin-4 does not accumulate following YM201636 treatment and undergoes negligible endocytosis.

<p>(A) MDCK cells treated with YM201636 showed no detectable accumulation of internal claudin-4. Scale bars 10 µm. (B) A surface biotinylation assay was performed to measure the endocytosis of claudin-4 and a Western blot from a representative experiment is shown. (C) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0028659#s2" target="_blank">Results</a> represented graphically show the mean from three independent experiments, error bars are SEM. Claudin-4 was successfully biotinylated (Surface Biotinylated) but negligible claudin-4 was internalised (Endocytosis 60 min) suggesting that claudin-4 is endocytosed at a much lower rate than claudin-1 and claudin-2.