Claudin-4 undergoes increased proteosomal degradation in EP2A cells.

<p>(A) EP2A cells grown as monolayer on culture plates were exposed with 20 µM of MG-132 (proteosomal inhibitor) for 10 h. Cells were collected and checked for claudin-4 expression by western blotting. Actin used as internal control protein. (B) The corresponding densitometry analysis for claudin-4 expression, ★ p<0.05. (C) Western blots depicting dose and time response of MG-132 treatment on claudin-4 expression in vector control, EP2S and EP2A cells. Two different doses of MG-132 (10 and 20 µM) exposed for 2, 4 and 10 h time period were studied. Control cells were exposed with only DMSO (diluent for MG-132) for 10 h. V = vector control; S = EP2S; A = EP2A.