IL-1β+IFN-γ modifies BCL2A1 and BMF expression.

<p>(A, B) RNA-seq data were validated for <i>BCL2A1</i> and <i>BMF</i> by qRT-PCR in 5 independent human islet preparations exposed or not (CTL) to the cytokines IL-1β+IFN-γ (CYT). Data were normalized to expression levels of the housekeeping gene <i>β-actin</i>. (C) INS-1E cells were left untreated or treated with IL-1β+IFN-γ for different times. <i>BCL2A1</i> expression was assayed by qRT-PCR and normalized for <i>β-actin</i> expression. The results are means ± SEM of 4 independent experiments. (D, E) INS-1E cells were transfected with control siRNA (siC, black bars) or either a single or a combination of 4 siRNAs (smart pool, sp) targeting <i>BCL2A1</i> (siBCL2A1, grey bars). After 48 h, cells were exposed to IL-1β+IFN-γ (CYT) for 16 h. (D) <i>BCL2A1</i> expression was measured by qRT-PCR and normalized for <i>β-actin</i> expression. Results are mean ± SEM of 4 independent experiments. (E) Apoptosis was examined by fluorescence microscopy after staining with the DNA-binding dyes propidium iodide and Hoechst 33342. Results are mean ± SEM of 4 independent experiments. *p<0.05, **p<0.01 versus untreated control (CTL); p<0.05 for the comparison siC versus siBCL2A1 as indicated.