IFNγ, Il-1β, and TNFα-induced changes in H3K4me1 enrichment correspond to changes in islet transcription.

<p>(A) Scatter plot of the FPKM of identified transcripts in untreated or IFNγ, Il-1β, and TNFα (Cytokine) treated islets. The relative expression of transcripts in the IFNγ, Il-1β, and TNFα (Cytokine) treated islets as compared to in untreated islets is indicated by the colour of the dot, with darker blue indicating higher expression in IFNγ, Il-1β, and TNFα treated islets, and darker red indicating higher expression in untreated islets. Only transcripts with an FPKM of >2 in one of the samples are shown. (B) Enrichment level (left graph) and p-values (right graph) of gene ontology (GO) terms enriched using genes identified as upregulated or down regulated by IFNγ, Il-1β, and TNFα exposure. (C) Violin plots of the expression level (FPKM) of transcripts in untreated (Unt) and IFNγ, Il-1β, and TNFα (Cytokine) treated islets associated with H3K4me1 enriched loci in each class (<i>de novo</i>, increased, unaltered, decreased). (D) Bar graphs of the fraction of H3K4me1 enriched loci in each class (<i>de novo</i>, increased, unaltered, decreased) associated with transcripts up or down regulated as indicated by IFNγ, Il-1β, and TNFα exposure. (E) Bar graphs of the fraction of transcripts up or down regulated by IFNγ, Il-1β, and TNFα exposure with associated <i>de novo</i>, increased (Incr), unaltered (Unalt), decreased (Decr) loci. (F) UCSC genome browser view of the genomic region around <i>Cxcl2</i>. RNA-seq reads and H3K4me1 enrichment data from IFNγ, Il-1β, and TNFα treated islets is shown in red, from untreated islets in blue, while H3K27me3 data from untreated islets is shown in black. ChIP-seq tracks are set to show a coverage depth range of 0 to 30, RNA-seq tracks from 0 to 500.