In vitro binding assay with [³H]PiB.

<p>Specific [³H]PiB binding to mouse and human brain homogenates and nonlinear modeling of data. (<b>A</b>) Binding isotherms for [³H]PiB with transgenic mouse brain tissues and human AD tissue containing Aβ deposits. Solid black curves show nonlinear fits with a single site model. Dashed lines describe 95% confidence bands around the fit. Resulting in vitro binding potential (BP) values and Aβ protein levels of these tissues are described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031310#pone-0031310-t003" target="_blank">Table 3</a>. (<b>B</b>) Semilogarithmic representation of the specific binding data as seen in panel (A) to delineate possible infliction points. (<b>C</b>) Scatchard graphs showing the same data as panel (A). Each data point is derived from the mean value of the original data octuples. Data show representative samples from tgtg-old (<i>red</i>), tgtg-young (<i>yellow</i>) and tg-old (<i>orange</i>) transgenic study group and severely affected human AD (huAD-C) tissue (<i>green</i>).