Biofilm formation by WT UA159, <i>glmS</i> mutant, and <i>nagB</i> mutant cells.

<p>After washing overnight cultures of WT and mutant cells, a small aliquot of each was inoculated into each of four solutions: (A) CDM-G50 with 50 mM sucrose, (B) CDM-G50 with 50 mM sucrose and 10 mM GlcNAc, (C) CDM containing 50 mM sucrose (no glucose), and (D) CDM containing 50 mM sucrose and 10 mM GlcNAc (no glucose). The cultures were then incubated at 37°C with 5% CO₂. After 24 h, the OD₆₆₀ was measured (lower panel). After washing the wells with PBS several times, the cells were stained with 0.1% safranin. The OD₄₉₀ was measured to quantify biofilm formation (upper panel). All experiments were performed in triplicate. *<i>p</i> < 0.05, compared to WT UA159 as determined by a <i>t</i>-test.