Sema 3A overexpression attenuates melanoma metastasis.

<p>(<b>A</b>) The mice described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033633#pone-0033633-g006" target="_blank">Fig. 6A</a>, were dissected ventrally and photographed. Growth of melanoma tumors were indicated by arrows (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033633#pone-0033633-g007" target="_blank">Fig. 7A</a>, panel I). Internal organs (liver, intestine and kidney) were analyzed histopathologically and the micrographs were shown at 60× magnification (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033633#pone-0033633-g007" target="_blank">Fig. 7A</a>, panels II–IV). Melanoma positive cells in metastatic lesions were marked by red arrows, whereas surrounding stromal regions were indicated by black arrows. Six mice were used in each group. Un-injected mice are used as control. (<b>B</b>) Sema 3A attenuates melanoma metastasis in mice. Control or clone 2 cells were injected intracardiaclly and mice were sacrificed, dissected ventrally and photographed (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033633#pone-0033633-g007" target="_blank">Fig. 7B</a>, panel I). Metastasized organs were indicated by arrows. Liver and intestine of the mice were analyzed histopathologically by H&E staining (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033633#pone-0033633-g007" target="_blank">Fig. 7B</a>, panels II & III). Micrographs were taken at 10× magnification. Arrows showed the melanoma foci. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033633#pone-0033633-g007" target="_blank">Fig. 7B</a>, panel IV shows the excised lung of respective mice. Lung sections were also analyzed histopathologically and photographs were taken at 10× magnification (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033633#pone-0033633-g007" target="_blank">Fig. 7B</a>, panel V). Melanoma metastatic foci in lung sections are indicated by arrows. Six mice were used in each set of experiment. Un-injected mice were used as control.