miR-34a inhibits pulmonary metastasis of osteosarcoma in vivo.

<p>(<b>A</b>) Representative macroscopic pictures of mouse lungs, 42 days after inoculation. (<b>B</b>) Graph displaying the total number of tumor nodules per lung in control group (stable SOSP-9607 cells transfected with pcDNA3.1) and miR-34a group (stable SOSP-9607 cells transfected with pcDNA-miR34a.). Data are presented as means±SD. *P<0.01 (n = 6) is considered as statistically significant. (<b>C</b>) Representative photographs of H&E stained spontaneous lung metastases. The results showed that most of the tumor nodules were organized in a predominantly peripheral distribution in both groups. However, tumor nodules (arrows) in miR-34a group were smaller and distributed in a lower concentration as comparing with control group.