Tumor growth and metastasis associated with miR-423-3p <i>in vivo</i>.

(A–B) Levels of miR-423-3p in five NSCLC cell lines (left panel), stable miR-423-3p-overexpression H1299 cells (Lenti-Mimic), and control H1299 cells (Lenti-Control) (right panel). (C) Tumor volumes in mice subcutaneously implanted with stable miR-423-3p-overexpression H1299 cells (left panel), and representative images of endpoint tumor burden, as determined by in vivo luciferase imaging (right panel). (D) Representative H&E-stained images and Ki-67 IHC-stained images of the miR-423-3p-overexpression xenograft. (E) Representative images of tumor burden, as determined by in vivo luciferase imaging of the lung, brain, liver, adrenal gland, and bone. Endpoint H&E staining results for mice subjected to the tail vein injection of stable miR-423-3p-overexpression H1299 cells. Data are presented as the means ± SD of three experiments. ** P P < 0.05.