PDGFRA represents the most functionally consequential miR -34a target.

<p><b>A</b>) TS543 cells were transfected with either miR-34a or control oligonucleotides for 24 hours, and NOTCH1 protein levels were measured by immunoblotting. <b>B</b>) 48 hours post-transfection, TS543 cells transfected with either miR-34a, siRNAs against PDGFRA, siRNAs against NOTCH1, or control oligonucleotides were pulsed with BrdU and assayed for cell proliferation by S-phase BrdU incorporation. siRNAs against PDGFRA and NOTCH1 are effective at reducing PDGFRA and NOTCH1 protein levels, respectively, as measured by immunoblotting 24 hours post-transfection. Error bars represent standard deviation within a single experimental set containing multiple replicates. Similar results were obtained in at least 3 independent experiments. *, P<0.05. **, P<0.005.