IPA analysis of differentially expressed genes in Leydig cells of GRTH^−/− compared to WT mice.

<p><b>(A) and (B). Left panel-</b>Venn diagram analysis of the overlap among differentially regulated genes in Leydig cells (144 down - and 155 up-regulated genes), in total testicular polysomes (307 down - or 53 up-regulated genes) and polysomal GRTH IP genes noted in WT mice (792 genes). Tpoly3fd or Tpoly3fu: >3 fold (f) down (d) or up (u)- regulated genes found in testicular polysomes (tpoly); lc3fd or lc3fu: >3 fold down- or up-regulated genes found in Leydig cells (lc); GRTHIP: mRNA messages were immunoprecipitated (IP) by GRTH antibody in testicular polysomal fraction of wild type mouse testis. <b>Right panel.</b> IPA predicts one network (nt) function in (<b>A</b>) 18 down- and (<b>B</b>) 3 up- regulated genes (A, in red) associated with GRTH protein in polysomes of Leydig cells. ltp: Leydig cells (l), testicular polysomes (t) and GRTH IP (p). Genes in color green (down-regulated), red (up-regulated) and uncolored (relevant biological genes to the network with no change in expression between WT and GRTH^−/−).