Tissue distribution and developmental expression patterns of germ cell-specific genes expressed in F9 cells.

<p>A. The expression patterns of nine genes predicted to be testis-specific or -predominant, as assessed in testis, F9 cells and NIH3T3 cells. Glyceraldehyde-3-phosphate dehydrogenase (<i>Gapdh</i>) was included as a loading control. Except for <i>Cpvl</i>, all of the genes were detected in both testis and F9 cells. It should be noted that the two bands for <i>Dmrt1</i> in NIH3T3 cells are non-specific, based on <i>in silico</i> investigation and an additional PCR analysis (data not shown). B. The tissue distributions of transcripts were assessed by RT-PCR analysis in various tissues of adult male mice. Complementary DNAs from various mouse tissues were amplified by PCR, with <i>Gapdh</i> included as a loading control. Seven genes were found to be testis-specific or -predominant. C. Developmental expression patterns during spermatogenesis. The stage-specific expression of the genes was determined from mouse testes on different days after birth (days 8, 10, 12, 14, 16, 20, 30, and 56). Abbreviations: <i>PL</i>, preleptotene; <i>L</i>, leptotene; <i>Z</i>, zygotene; <i>P</i>, pachytene; <i>D</i>, diplotene; <i>MI</i>, meiotic division I; and <i>MII</i>, meiotic division II. Complementary DNA from germ cell-lacking testes from </p><p>W/W^v</p> mutant mice was also examined. Consistent with our microarray analysis, <i>Tex13</i>, <i>Stra8</i>, <i>Fancd2os</i>, 1700061G19Rik and <i>Triml1</i> were found to be germ cell-specific.<p>