Physical interaction between TRα1 and the β-catenin/Tcf4 complex.

<p>Nuclear extracts from Caco2 cells, maintained in T3-depleted (−) or T3-supplemented (+) serum, were immunoprecipitated with antibodies directed against endogenous Tcf4, β-catenin or TRα1 and analyzed by WB by using the antibodies as indicated. Rabbit IgG was used as negative control. Ponceau red was used as whole-protein (50 µg/lane) loading control. Histone H1 was used to check the enrichment and was the loading control for the nuclear proteins in the inputs. The pictures are representative of at least three independent experiments.