Loss of <i>Capr</i> disrupts germline cyst development.

<p>A) Schematic of the <i>Drosophila</i> germarium with bars below indicating the numbered germarium regions (1, 2a, 2b, 3) and their cell types: non-proliferating terminal filament (TF) and cap cells (CC), germline stem cells (GSC) which give rise to the developing 16-cell cysts (cyst), escort cells (EC) which facilitate movement of cysts through regions 1 and 2a, and the follicle stem cells (FSC) which give rise to the follicle cells (FC) and stalk. Anterior is to the left in all figures. B-C) Immunofluorescence analysis of control <i>+/Df(3L)Cat</i> (B, B’) or <i>Capr²/Df(3L)Cat</i> (C, C’) germaria stained with antibodies to Slit (green and B’, C’) and TO-PRO-3 iodide (DNA, red). Arrowheads indicate the position of FSCs and the unencapsulated cysts are numbered in B’ and C’. Scale bar is 30 microns.