Loss of Lethal giant discs induces the formation of stem-cysts and 32-cell egg chambers.

<p>(A) Female germline clones (GLC) of <i>lgd^d7</i> (<i>hsFlp/+; lgd^d7, FRT 40A/ GFP, FRT 40A</i>) stained with DAPI (DNA, blue) and phalloidin (F-actin, red). Egg chamber with heterozygous germ cells (GFP, green) is made of 16 cells with a 4 ring canals oocyte (close up in A’). The neighboring egg chamber is a GLC, made of 32 cells with a 5 ring canals oocyte (close up in A’’). (B) Fraction of egg chambers exhibiting 32 cells on the y axis. Genotypes are on the x axis. (C) Female germline clones (GLC) of <i>lgd^d7</i> stained for DAPI (DNA, blue) and α-spectrin (fusome, red). A stem-cyst composed of 5 homozygous mutant cells is shown. (D) Fraction of germaria exhibiting at least one stem-cyst on the y axis. Genotypes are on the x axis. Scale bar: 10 μm.