NK cell depletion results in loss of neutralization against a CRF01_AE virus when using a ICp24 assay format.
- Bruce K. Brown (171729)
- Lindsay Wieczorek (171734)
- Gustavo Kijak (171738)
- Kara Lombardi (171744)
- Jeffrey Currier (171750)
- Maggie Wesberry (171757)
- John C. Kappes (161603)
- Viseth Ngauy (171761)
- Mary Marovich (171767)
- Nelson Michael (171773)
- Christina Ochsenbauer (161594)
- David C. Montefiori (108747)
- Victoria R. Polonis (132293)
Publication date
April 2012
DOIAbstract
<p>Neutralization was calculated employing a subtype CRF01_AE IMC (virus CM235) without a reporter gene inserted and using a flow cytometric intracellular p24 endpoint (panels A–D). The ID<sub>50</sub>s for 2 polyclonal antibody pools (A, C) or IC<sub>50</sub>s for 4E10 (B, D) are displayed for PBMC from 2 donors that were used either in bulk (black bars) or after NK cell depletion (white bars).</p
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