Ca²⁺ response elicited by γEVG involves intracellular Ca²⁺ stores and phospholipase C.

<p>(A) γEVG (100 µM) was focally applied in medium containing Ca²⁺ (left trace) or in the absence of Ca²⁺ (Ca-free medium with 0.2 mM EGTA; right trace). (B) Responses elicited by depolarization (bath-applied KCl, 50 mM) and influx of Ca²⁺ through voltage-dependent Ca²⁺ channels were abolished in the absence of extracellular Ca²⁺. (C) Mean amplitudes of responses in the presence or absence of Ca²⁺ in the medium (mean ± SE; *<i>p</i>≤0.05, n = 4 cells). (D) Responses to γEVG inhibited by U73122 (10 µM). (E) Mean amplitudes of the responses in the presence or absence of U73122 (mean ± SE; *<i>p</i>≤0.05, n = 4 cells).