Comparative assay for the effects of extracellular Ca²⁺ on 2MeS-ATP-induced intracellular Ca²⁺-mobilization in H295R.

<p>A 1000 µM aliquot of 2MeS-ATP was applied to the cells under the extracellular Ca²⁺ (1.2 mM Ca²⁺) existence or Ca²⁺-free (with 2 mM EGTA) conditions. The peak ratios of the fluorescent intensity (F₃₄₀/F₃₈₀) and the τ by the agonist represented in Mean±SD (N = 14) were: extracellular 1.2 mM Ca²⁺, 0.64±0.123 and 7.42±2.78 min; extracellular Ca²⁺-free, 0.58±0.164 and 2.82±1.09 min, respectively. The difference between the τ from the two conditions was significant (<i>p</i><0.001).