Co-immunoprecipitation of P3N-PIPO and PCaP1 expressed <i>in planta</i>.

<p>Proteins from crude extracts of <i>N. benthamiana</i> leaves (2 days post agroinfiltration) that co-expressed HA-P3N-PIPO and c-myc-PCaP1, or expressed HA-P3N-PIPO or c-myc-PCaP1 only were pulled-down using anti-HA (top panels) or anti-c-myc (bottom panels) antibodies, separated by 4–12% Novex Tris-Glycine PAGE, electroblotted onto PVDF membrane and probed with anti-HA or anti-c-myc antibody as indicated. Recognition of HA-P3N-PIPO and c-myc-PCAP1 are shown at right. Immunoblotting (IB).