Identified proteins interact <i>in vivo</i> with DBP1.

<p>A, BiFC assay with YFP fusions transiently expressed in <i>N. benthamiana</i> leaves by agroinfiltration. Both reciprocal combinations are shown for each interaction. As a negative control, DBP1 and the unrelated transcription factor BREVIS RADIX (BRX) YFP fusions were used. Centromeric histone H3 fused to mRFP was co-expressed as a marker for nuclear localization. B, Coimmunoprecipitation validated the reported interactions. Relevant fusion proteins were transiently expressed in <i>N. benthamiana</i> leaves by agroinfiltration as indicated, and immunoprecipitated with anti-MaBP antibodies. Western blots of the immunoprecipitated fractions decorated with the indicated antibodies are shown. Anti-GFP antibodies allow for detection of YFP.