<i>P. aeruginosa</i> PAO1 response to k-opioid during growth in nutrient poor medium.

<p>(<b>A</b>) Effect of 200 µM U-50,488 on PAO1 cell density, n = 3. (<b>B</b>) Pyoverdin production normalized to cell density, n = 3, *p<0.01. (<b>C</b>) Pyocyanin production normalized to cell density, n = 3, *p<0.05. (<b>D–D″</b>) Transmission electron microscopy images of PAO1 cells grown in (<b>D</b>) 0.1xTY, and (<b>D′, D″</b>) grown in 0.1xTY supplemented with 200 µM U-50,488. Flagella (<b>D′</b>) and vesicles (<b>D″</b>) are shown by arrows. (<b>E</b>) Kaplan-Meier survival curves in <i>C. elegans</i> feeding in <i>P. aeruginosa</i> PAO1 grown in poor nutrient medium vs poor nutrient medium containing 50 µM U-50,488. Cell cultures were collected at 7 hrs. Cumulative survival is represented as n = 7/plate, 3 plates/experiment, p<0.01.