P1 and P2 phenotypes of <i>P. aeruginosa</i> MPAO1.

<p>(A) Pyocyanin production seen as green color pigmentation on solid PIA and liquid TSB media. (B) Kaplan-Meyer survival curves of <i>C. elegans</i> N2 feeding on P1 and P2. Cumulative survival is represented of 2 experiments, n = 7/dish, 5 dishes/experiment, p<0.01. (C) Swarming motility. (D) Wound healing assay. Wound width was calibrated and measured using the MicroSuite software for imaging applications (Olympus SZX16). Wound healing of −100% indicates a 2 fold increase in the wound width compared to the baseline width. n = 12, *p<0.01. (E,F) Collagenase activity of P1 and P2 measured by degradation of fluorescent labeled collagen I (E) and collagen IV (F) as substrates. n = 6, *p<0.01. Fluorescence values were normalized to cell density measured by absorbance at 600 nm. Results are representative of 3 independent experiments. (G) RAPD fingerprint analysis demonstrating a similar genetic background of the P1 and P2 phenotype strains.