Characterization of three <i>jmj18</i> mutants.

<p>(A) Structures of <i>JMJ18</i> and its gene product. Arrows indicate the T-DNA insertion sites in the mutants. Introns are represented as lines, exons as filled black boxes, and untranslated regions as filled gray boxes. The primers used for transcript detection (P1–P3) are indicated under the schematic gene structure. (B) Transcript analysis of the <i>jmj18</i> mutants by RT-PCR. Pairs of P1/P3 and P1/P2 were used for full-length and partial transcripts detection, respectively. The 11-day-old plants of each genotype were collected at dusk for RNA isolation. The <i>jmj18</i> mutants exhibited a weak late-flowering phenotype under both LD (C) and SD (D) conditions. Bar = 2 cm. Relative expression levels of <i>FLC</i> (E), <i>CO</i> (F), and <i>FT</i> (G) in the <i>jmj18</i> mutants. The expression level was normalized to that of <i>ACTIN</i>. RNA was isolated from eleven-day-old seedlings. The values are the mean and standard deviation from three independent experiments. There was no significant difference between wild-type and <i>jmj18</i> mutants for the expression levels of <i>FLC</i> (E), <i>CO</i> (F) or <i>FT</i> (G) tested by Student's <i>t</i> test (P<0.05).