The JmjN, JmjC, and zinc-finger domains are necessary for the promotion of flowering by JMJ18.

<p>(A) Schemes display the structures of full-length JMJ18 protein as well as truncated versions used for overexpression test. (B) and (C) Flowering time phenotype for the <i>JMJ18</i> overexpressor lines. All of the plants in (B) were grown for 24 days under LD conditions. All of the 108 independent <i>35S:NC</i> lines examined flowered at a similar time compared to wild type. Fifteen out of 108 <i>35S:NCZ</i>, 20 out of 108 <i>35S:T</i>, and 27 out of 108 <i>35S:JMJ1</i>8 lines displayed an early-flowering phenotype. Two independent lines per transformation were shown. Bar = 2 cm. The values in (C) are the mean ± standard deviation. At least 15 plants were scored for each genotype. Asterisks indicate the significant difference between wild-type and transgenic plants analyzed by Student's <i>t</i> test (P<0.05) using rosette leaf numbers. (D) Northern blot analysis of the expression of truncated <i>JMJ18</i> in the transgenic lines.