Spermatogenesis defects in <i>spe-44</i> males.

<p>A–B. Isolated gonads from wild-type (A) and <i>spe-44</i> (B) males visualized by DIC. Indicated are pachytene (p), karyosome (k), and meiotically dividing (d) spermatocytes, and spermatids (sp). C–E. Sperm spreads from wild-type (C) and <i>spe-44</i> (D–E) males. C. Wild-type spermatogenesis with spermatocytes in anaphase I (ana I), anaphase II (ana II), post-meiotic budding division (bd), and mature spermatids (sp). D. <i>spe-44</i> spermatogenesis with aberrantly dividing spermatocytes. E. Enlarged image of <i>spe-44</i>. F–G. Isolated gonads from wild-type (F) and <i>spe-44</i> (G) males co-stained with anti-tubulin (green) and DAPI (blue). Labeling as in panels A–B. Additionally indicated are residual bodies from the budding division (bd), and a mix of terminally arrested and dying spermatocytes (*). H. Higher magnification of individual sperm from <i>spe-44</i> males. Top row, DAPI staining to highlight nuclear morphologies; bottom row, merge of DAPI (blue) and tubulin (green). See text for labeling.