TcCPR27 and TcCPR18 are abundant in cuticle of the pronotum.
- Yasuyuki Arakane (168375)
- Joseph Lomakin (168380)
- Stevin H. Gehrke (168385)
- Yasuaki Hiromasa (122247)
- John M. Tomich (122260)
- Subbaratnam Muthukrishnan (116973)
- Richard W. Beeman (116971)
- Karl J. Kramer (116969)
- Michael R. Kanost (5123)
Publication date
April 2012
DOIAbstract
<p>To suppress cuticle tanning, dsRNA for <i>TcLac2</i> (2 ng per insect) was injected into day 0 pupae. Proteins from the pronotum and elytra were extracted from five adults at 0–30 min after eclosion, and the extracts were analyzed by SDS-PAGE (left panel). In the ds<i>Ver</i>-treated control animals, the extent of tanning of the pronotum was substantially greater than that of the elytron (right panel). S: PBS homogenate supernatant, P: PBS homogenate pellet.</p
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