The D7H mutation promotes Aβ40 HMW assemblies and Aβ42 LMW assemblies formation.
- Wei-Ting Chen (167673)
- Chen-Jee Hong (160996)
- Ya-Tzu Lin (167674)
- Wen-Han Chang (129553)
- He-Ting Huang (167675)
- Jhih-Ying Liao (167676)
- Yu-Jen Chang (167677)
- Yi-Fang Hsieh (167678)
- Chih-Ya Cheng (160978)
- Hsiu-Chih Liu (167679)
- Yun-Ru Chen (54058)
- Irene H. Cheng (167685)
Publication date
April 2012
DOIAbstract
<p>Lyophilized Aβ was prepared in GdnHCl for the ThT assay (A, B) or in HFIP-DMSO for Western blot (C, D), and samples were collected at indicated times. (A, B) The ThT assay was applied to monitor the kinetics of β-sheet formation for Aβ40<sub>wt</sub> (A,•), Aβ40<sub>D7H</sub> (A,○), Aβ42<sub>wt</sub> (B,•) and Aβ42<sub>D7H</sub> (B,○). Data were averaged from 3–4 independent experiments (n = 3 per experiment). (C) Aβ40 and (D) Aβ42 samples were fixed by PICUP and examined by Western blot to analyze the size distribution of assemblies during aggregation.</p
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