CSPα oligomerization is greater in CSPα_L115R, CSPα_Δ116 mutants.

<p><b>(A)</b> CAD cells were transiently transfected with 0.75 μg cDNA encoding myc tagged wild type CSPα or 1.0 μg cDNA encoding either CSPα_L115R, CSPα_L116Δ or CSPα_HPD-AAA as indicated and lysed 48 hours post-transfection. CSPα monomer and oligomers were detected in CAD cell lysates by western blot with anti-myc (upper panel) and anti-CSPα (middle panel). Detection of β-actin was used to verify equal loading amongst the various lanes. <b>(B)</b> Quantification of CSPα high molecular weight oligomers (starting from 70kDa) CSPα monomers. *p < 0.01. Results are from 4 independent experiments.