UT activation mediating GABA_AR internalization.

<p>(Aa–Ad) CHO-UT transiently transfected with cDNA encoding α₂β₃^HAγ₂ GABA_AR subunits. Internalization was controlled through translocation of β₃^HA subunit (red) in control (Aa) or after 60 min of <i>h</i>UII (10⁻⁸ M, Ab), Iso (10⁻⁴ M, Ac) or <i>h</i>UII+Iso (Ad) incubation. Fluorescence intensity plots of green and red fluorescences corresponding to the localization of GABA_AR (β₃^HA) at the plasma membrane and in the cytosol, respectively, across the regions delimited by the white line scans. A.u., arbitrary unit; scale bars, 25 µm. (B) Bar graphs of the fraction of fluorescence at the plasma membrane on CHOT-UT-GABA_AR or CHO-GABA_AR in the different conditions. Each bar corresponds to mean ± SEM percent obtained from 3 to 18 cells. ns, non significant; ***, <i>P</i><0.001 <i>versus</i> control in CHO-UT-GABA_AR; ###, <i>P</i><0.001 <i>versus</i> control in CHO-GABA_AR.