YFPγ2 fluorescence correlates with immunostaining for GABA_AR α subunits.

<p>Cross-correlation analysis of vertical sections from P12 and P21 <i>Thy1-YFPγ2</i> retinas stained with anti-GABA_AR α1 (<b>A–D</b>), GABA_AR α2 (<b>E–H</b>), or GABA_AR α3 (<b>I–L</b>). (<b>M</b>) Image stack of a single ON RGC labeled by transfection of CMV:tdTomato in a <i>Thy1-YFPγ2</i> retina, immunostained for GABA_AR α3. (<b>N</b>) The dendritic label was used to mask out the YFPγ2 and GABA_AR α3 fluorescence. (<b>O</b>) (Upper) YFPγ2 and GABA_AR α3 puncta within sections of dendritic arbors identified by custom-written software. (Lower) Fraction of the population of YFPγ2 puncta that colocalized with GABA_AR α3, and vice-versa (average and SEM of 4 cells). White arrowheads are examples of colocalized puncta, green and red arrowheads, non-colocalized puncta. Scale bars 5 µm.